Recombinant:Article Title: Genomic copy-number variants drive apoptotic evasion underlying acquired resistance to immune checkpoint inhibitors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies beta-2 Microglobulin Monoclonal Antibody (B2M-02) Invitrogen Cat# MA1-19413; RRID: AB_1070703 GSDMC Polyclonal Antibody Invitrogen Cat# PA5-116594; RRID: AB_2901225 Fas Recombinant Rabbit Monoclonal Antibody (JJ0942) Invitrogen Cat# MA5-32489; RRID: AB_2809766 DFFB Polyclonal Antibody Invitrogen Cat# PA5-115114; RRID: AB_2899750 Caspase 9 Monoclonal Antibody (LAP6 96-2-22) Invitrogen Cat# MA1-16842; RRID: AB_568482 Bad Antibody Cell Signaling Technology Cat# 9292; RRID: AB_331419 alpha-Tubulin Antibody Cell Signaling Technology Cat# 2144; RRID: AB_2210548 Bax (E4U1V) Rabbit mAb Cell Signaling Technology Cat# 41162; RRID: AB_2924730 DYKDDDDK Tag (D6W5B) Rabbit mAb Cell Signaling Technology Cat# 14793; RRID: AB_2572291 p53 (7F5) Rabbit mAb Cell Signaling Technology Cat# 2527; RRID: AB_10695803 TNF-R1 (C25C1) Rabbit mAb Cell Signaling Technology Cat# 3736; RRID: AB_2241018 TAP1 (E4T4F) Rabbit mAb Cell Signaling Technology Cat# 49671 TAP2 (E8G5I) Rabbit mAb Cell Signaling Technology Cat# 25657 IFNGR1 Antibody Cell Signaling Technology Cat# 34808; RRID: AB_2799061 FITC anti-human CD3 Antibody BioLegend Cat# 317306; RRID: AB_571907 PE anti-human TCR Vβ13.1 Antibody BioLegend Cat# 362410; RRID: AB_2750159 Goat anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM Plus 488 Invitrogen Cat# A32723; RRID: AB_2633275 Goat anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 555 Invitrogen Cat# A-21429; RRID: AB_2535850 Bacterial and virus strains NEB® Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H Biological samples Tumor and patient-matched normal tissues University of California, Los Angeles, Sutter Health, and the Vanderbilt-Ingram Cancer Center See Table S1 for details Peripheral blood mononuclear cells (PBMCs) UCLA virology core laboratory N/A Mouse syngeneic tumors Dr. Roger Lo N/A Chemicals, peptides, and recombinant proteins Anti-Mouse CD279 (PD-1) (Clone RMP1-14) Leinco Tech Cat# P362 InVivoMAb anti-mouse CTLA-4 (CD152) BioXcell Cat# BE0131 Venetoclax TargetMol Cat# T2119 PEG300 MedChemExpress Cat# HY-Y0873 Antigen Retrieval Buffer Abcam Cat# ab93684 DAPI MilliporeSigma Cat# D9542 Molecular ProbesTM ProLongTM Diamond Antifade Mountant Thermo Fisher Scientific Cat# P36965 BioT Bioland Scientific Cat# B01-00 (Continued on next page) Immunity 58, 1–14.e1–e9, November 11, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium butyrate Thermo Fisher Scientific Cat# A11079.36 HEPES Thermo Fisher Scientific Cat# 15630080 Recombinant human IL-2 Miltenyi Biotec Cat# 130-097-748 DynabeadsTM Human T-Activator CD3/ CD28 for T Cell Expansion and Activation Gibco Cat# 11161D RetroNectin Takara Cat# T110A Cryostor CS10 cryopreservation medium Biolife Solutions Cat# 210102 G418 disulfate salt MilliporeSigma Cat# A1720-1G Blasticidin S HCl Gibco Cat# R21001 Puromycin MilliporeSigma Cat# P8833-25MG Hygromycin B Thermo Fisher Scientific Cat# AAJ6068103 Polybrene Infection / Transfection Reagent MilliporeSigma Cat# TR-1003-G RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89901 HaltTM Phosphatase Inhibitor Single-Use Cocktail Thermo Fisher Scientific Cat# 78428 PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23227 Tween 80 Thermo Fisher Scientific Cat# BP338-500 4% Paraformaldehyde in PBS Thermo Fisher Scientific Cat# J61899.AP Crystal violet solution MilliporeSigma Cat# V5256 Tris-HCl (PH7.4) MilliporeSigma Cat# T2194 Tween 20 Andwin Scientific Cat# NC9022994 Nonidet P40 Substitute MilliporeSigma Cat# 74385 NaCl Invitrogen Cat# AM9760G MgCl2 Invitrogen Cat# AM9530G Triton X-100 Thermo Fisher Scientific Cat# ICN19485483 QIAGEN Protease (30 AU) Qiagen Cat# 19157 0.5M EDTA Thermo Fisher Scientific Cat# R1021 Xylene Fisher Scientific Cat# X5-1 Ethyl Alcohol Sigma-Aldrich Cat# E7023 Critical commercial assays QIAGEN AllPrep DNA/RNA Mini Kit Qiagen Cat# 80204 QIAGEN QIAamp DNA FFPE Tissue Kit Qiagen Cat# 56404 QIAGEN FlexiGene DNA Kit Qiagen Cat# 51206 Pan T Cell Isolation Kit Miltenyi Biotec Cat# 130-096-535 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat# 130-096-730 FFPE Tissue Dissociation Kit Miltenyi Biotec Cat# 130-118-052 Illumina Tagment DNA Enzyme and Buffer Large Kit Illumina Cat# 20034198 KAPA HotStart PCR Kit, with dNTPs Roche Cat# KK2502 Qubit dsDNA HS (High Sensitivity) Assay Kit Invitrogen Cat# Q32851 Qubit dsDNA BR (Broad-Range) Assay Kit Invitrogen Cat# Q32850 Deposited data WES This paper European Genome-phenome Archive (EGA): EGAS50000001055 WGS This paper EGA: EGAS50000001055 WES Zaretsky et al. 15 SRA: SRP076315 WES Hugo et al. 11 SRA: SRP067938, SRP090294 WES Liu et al., 13 Hugo et al., 44 Moriceau et al., 45 Shi et al. 46 SRA: SRP049746 Copy number profiles of TCGA-SKCM cohort TCGA, PanCancer Atlas https://www.cbioportal.org/ (Continued on next page) e2 Immunity 58, 1–14.e1–e9, November 11, 2025
Virus:Article Title: Genomic copy-number variants drive apoptotic evasion underlying acquired resistance to immune checkpoint inhibitors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies beta-2 Microglobulin Monoclonal Antibody (B2M-02) Invitrogen Cat# MA1-19413; RRID: AB_1070703 GSDMC Polyclonal Antibody Invitrogen Cat# PA5-116594; RRID: AB_2901225 Fas Recombinant Rabbit Monoclonal Antibody (JJ0942) Invitrogen Cat# MA5-32489; RRID: AB_2809766 DFFB Polyclonal Antibody Invitrogen Cat# PA5-115114; RRID: AB_2899750 Caspase 9 Monoclonal Antibody (LAP6 96-2-22) Invitrogen Cat# MA1-16842; RRID: AB_568482 Bad Antibody Cell Signaling Technology Cat# 9292; RRID: AB_331419 alpha-Tubulin Antibody Cell Signaling Technology Cat# 2144; RRID: AB_2210548 Bax (E4U1V) Rabbit mAb Cell Signaling Technology Cat# 41162; RRID: AB_2924730 DYKDDDDK Tag (D6W5B) Rabbit mAb Cell Signaling Technology Cat# 14793; RRID: AB_2572291 p53 (7F5) Rabbit mAb Cell Signaling Technology Cat# 2527; RRID: AB_10695803 TNF-R1 (C25C1) Rabbit mAb Cell Signaling Technology Cat# 3736; RRID: AB_2241018 TAP1 (E4T4F) Rabbit mAb Cell Signaling Technology Cat# 49671 TAP2 (E8G5I) Rabbit mAb Cell Signaling Technology Cat# 25657 IFNGR1 Antibody Cell Signaling Technology Cat# 34808; RRID: AB_2799061 FITC anti-human CD3 Antibody BioLegend Cat# 317306; RRID: AB_571907 PE anti-human TCR Vβ13.1 Antibody BioLegend Cat# 362410; RRID: AB_2750159 Goat anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM Plus 488 Invitrogen Cat# A32723; RRID: AB_2633275 Goat anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 555 Invitrogen Cat# A-21429; RRID: AB_2535850 Bacterial and virus strains NEB® Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H Biological samples Tumor and patient-matched normal tissues University of California, Los Angeles, Sutter Health, and the Vanderbilt-Ingram Cancer Center See Table S1 for details Peripheral blood mononuclear cells (PBMCs) UCLA virology core laboratory N/A Mouse syngeneic tumors Dr. Roger Lo N/A Chemicals, peptides, and recombinant proteins Anti-Mouse CD279 (PD-1) (Clone RMP1-14) Leinco Tech Cat# P362 InVivoMAb anti-mouse CTLA-4 (CD152) BioXcell Cat# BE0131 Venetoclax TargetMol Cat# T2119 PEG300 MedChemExpress Cat# HY-Y0873 Antigen Retrieval Buffer Abcam Cat# ab93684 DAPI MilliporeSigma Cat# D9542 Molecular ProbesTM ProLongTM Diamond Antifade Mountant Thermo Fisher Scientific Cat# P36965 BioT Bioland Scientific Cat# B01-00 (Continued on next page) Immunity 58, 1–14.e1–e9, November 11, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium butyrate Thermo Fisher Scientific Cat# A11079.36 HEPES Thermo Fisher Scientific Cat# 15630080 Recombinant human IL-2 Miltenyi Biotec Cat# 130-097-748 DynabeadsTM Human T-Activator CD3/ CD28 for T Cell Expansion and Activation Gibco Cat# 11161D RetroNectin Takara Cat# T110A Cryostor CS10 cryopreservation medium Biolife Solutions Cat# 210102 G418 disulfate salt MilliporeSigma Cat# A1720-1G Blasticidin S HCl Gibco Cat# R21001 Puromycin MilliporeSigma Cat# P8833-25MG Hygromycin B Thermo Fisher Scientific Cat# AAJ6068103 Polybrene Infection / Transfection Reagent MilliporeSigma Cat# TR-1003-G RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89901 HaltTM Phosphatase Inhibitor Single-Use Cocktail Thermo Fisher Scientific Cat# 78428 PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23227 Tween 80 Thermo Fisher Scientific Cat# BP338-500 4% Paraformaldehyde in PBS Thermo Fisher Scientific Cat# J61899.AP Crystal violet solution MilliporeSigma Cat# V5256 Tris-HCl (PH7.4) MilliporeSigma Cat# T2194 Tween 20 Andwin Scientific Cat# NC9022994 Nonidet P40 Substitute MilliporeSigma Cat# 74385 NaCl Invitrogen Cat# AM9760G MgCl2 Invitrogen Cat# AM9530G Triton X-100 Thermo Fisher Scientific Cat# ICN19485483 QIAGEN Protease (30 AU) Qiagen Cat# 19157 0.5M EDTA Thermo Fisher Scientific Cat# R1021 Xylene Fisher Scientific Cat# X5-1 Ethyl Alcohol Sigma-Aldrich Cat# E7023 Critical commercial assays QIAGEN AllPrep DNA/RNA Mini Kit Qiagen Cat# 80204 QIAGEN QIAamp DNA FFPE Tissue Kit Qiagen Cat# 56404 QIAGEN FlexiGene DNA Kit Qiagen Cat# 51206 Pan T Cell Isolation Kit Miltenyi Biotec Cat# 130-096-535 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat# 130-096-730 FFPE Tissue Dissociation Kit Miltenyi Biotec Cat# 130-118-052 Illumina Tagment DNA Enzyme and Buffer Large Kit Illumina Cat# 20034198 KAPA HotStart PCR Kit, with dNTPs Roche Cat# KK2502 Qubit dsDNA HS (High Sensitivity) Assay Kit Invitrogen Cat# Q32851 Qubit dsDNA BR (Broad-Range) Assay Kit Invitrogen Cat# Q32850 Deposited data WES This paper European Genome-phenome Archive (EGA): EGAS50000001055 WGS This paper EGA: EGAS50000001055 WES Zaretsky et al. 15 SRA: SRP076315 WES Hugo et al. 11 SRA: SRP067938, SRP090294 WES Liu et al., 13 Hugo et al., 44 Moriceau et al., 45 Shi et al. 46 SRA: SRP049746 Copy number profiles of TCGA-SKCM cohort TCGA, PanCancer Atlas https://www.cbioportal.org/ (Continued on next page) e2 Immunity 58, 1–14.e1–e9, November 11, 2025
|
New England Biolabs is a verified supplier 
New England Biolabs manufactures this product 